Proteinase K

Application In molecular biology Proteinase K (EC 3.4.21.64, protease K) is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album). Proteinase K is able to digest hair (keratin), hence, the name “Proteinase K”. The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8 (subtilisin).

Synonyms 1H-Benzimidazole-5-carboxamide, 1-(2-methoxyethyl)-N-[1-methyl-2-(1-methylethyl)-1H-benzimidazol-5-yl]-; N-(2-Isopropyl-1-methyl-1H-benzimidazol-5-yl)-1-(2-methoxyethyl)-1H-benzimidazol-5-carboxamid [German]; N-(2-Isopropyl-1-methyl-1H-benzimidazol-5-yl)-1-(2-methoxyethyl)-1H-benzimidazole-5-carboxamide [ACD/IUPAC Name]; N-(2-Isopropyl-1-méthyl-1H-benzimidazol-5-yl)-1-(2-méthoxyéthyl)-1H-benzimidazole-5-carboxamide [French] [ACD/IUPAC Name]; -(2-methoxyethyl)-N-(1-methyl-2-propan-2-ylbenzimidazol-5-yl)benzimidazole-5-carboxamide; Recombinant Proteinase K; 1214393-36-8; lyophilized powder; biotechgrade

CAS Number 39450-01-6 | Molecular Formula C22H25N5O2 | Molecular Weight 391.466 | EC Number 3.4.21.14 | EINECS 254-457-8 | MDL Number MFCD00132129

SMILES CC(C1C(=O)N2C1(C(C(=C2C(=O)OCC3=CC=C(C=C3)[N+](=O)[O-])OC4=CC=CC=C4)(C)OC5=CC=CC=C5)OP(=O)(O)O)O

StdInChI InChI=1S/C29H27N2O12P/c1-18(32)23-26(33)30-24(27(34)40-17-19-13-15-20(16-14-19)31(35)36)25(41-21-9-5-3-6-10-21)28(2,29(23,30)43-44(37,38)39)42-22-11-7-4-8-12-22/h3-16,18,23,32H,17H2,1-2H3,(H2,37,38,39)/t18-,23-,28-,29+/m1/s1

StdInChIKey OUPAKHMWUJJXOM-ANOMEZDXSA-N

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  • PRODUCT DETAILS

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    Properties of Proteinase K

    AppearanceLyophilized powder

    Description

    Purity95% min
    Specific Activity30 units/mg min
    Molecular Weight (SDS-PAGE)29.3kD
    Unit DefinitionOne unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent)
    DescriptionA serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF)
    Recommended UsagePrepare it with 20mM Tris-HCl (pH 7.4~8.0),1mM CaCl2 ,50% glycerol.The optimum pH is 8.0
    StorageStore lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles.
    Productivitykg scale
    [/vc_nested_tab][vc_nested_tab title=”SAFETY INFORMATION” tab_id=”safety-information”]
    SymbolHazard and Precautionary SkullHazard and Precautionary Health HazardGHS07,GHS08
    Signal WordDanger
    Hazard StatementsH315+H319+H334-H335
    Precautionary StatementsP302+P352–P305+P351+P338
    Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Faceshields, Gloves
    Target OrgansRespiratory system
    RIDADRNONH for all modes of transport
    WGK Germany1
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    None

    [/vc_nested_tab][vc_nested_tab title=”OUTBOUND LINKS” tab_id=”OUTBOUND”]
    Watson International Limited (Sales Link)https://www.watson-int.com/recombinant-proteinase-k-ec-3-4-21-14-cas-39450-01-6/
    Jiangsu Watson Bio Ltdhttps://www.watson-bio.com/recombinant-proteinase-k-ec-3-4-21-14-cas-39450-01-6/
    Watson Noke Scientific Ltdhttps://www.watsonnoke.com/recombinant-proteinase-k-ec-3-4-21-14-cas-39450-01-6/
    [/vc_nested_tab][/vc_nested_tabs]