Application In molecular biology Proteinase K (EC 22.214.171.124, protease K) is a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album). Proteinase K is able to digest hair (keratin), hence, the name “Proteinase K”. The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8 (subtilisin).
Synonyms 1H-Benzimidazole-5-carboxamide, 1-(2-methoxyethyl)-N-[1-methyl-2-(1-methylethyl)-1H-benzimidazol-5-yl]-; N-(2-Isopropyl-1-methyl-1H-benzimidazol-5-yl)-1-(2-methoxyethyl)-1H-benzimidazol-5-carboxamid [German]; N-(2-Isopropyl-1-methyl-1H-benzimidazol-5-yl)-1-(2-methoxyethyl)-1H-benzimidazole-5-carboxamide [ACD/IUPAC Name]; N-(2-Isopropyl-1-méthyl-1H-benzimidazol-5-yl)-1-(2-méthoxyéthyl)-1H-benzimidazole-5-carboxamide [French] [ACD/IUPAC Name]; -(2-methoxyethyl)-N-(1-methyl-2-propan-2-ylbenzimidazol-5-yl)benzimidazole-5-carboxamide; Recombinant Proteinase K; 1214393-36-8; lyophilized powder; biotechgrade
CAS Number 39450-01-6 | Molecular Formula C22H25N5O2 | Molecular Weight 391.466 | EC Number 126.96.36.199 | EINECS 254-457-8 | MDL Number MFCD00132129
Properties of Proteinase K
Appearance Lyophilized powder
Purity 95% min Specific Activity 30 units/mg min Molecular Weight (SDS-PAGE) 29.3kD Unit Definition One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent) Description A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF) Recommended Usage Prepare it with 20mM Tris-HCl (pH 7.4~8.0),1mM CaCl2 ,50% glycerol.The optimum pH is 8.0 Storage Store lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles. Productivity kg scale
Watson International Limited (Sales Link) https://www.watson-int.com/recombinant-proteinase-k-ec-3-4-21-14-cas-39450-01-6/ Jiangsu Watson Bio Ltd https://www.watson-bio.com/recombinant-proteinase-k-ec-3-4-21-14-cas-39450-01-6/ Watson Noke Scientific Ltd https://www.watsonnoke.com/recombinant-proteinase-k-ec-3-4-21-14-cas-39450-01-6/